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2×rna gel loading dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 2×rna gel loading dye
    2×Rna Gel Loading Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+load+dye/Agarose+Gel+Loading+Dye/pm42236761-136-7-11
    Average 96 stars, based on 1 article reviews
    2×rna gel loading dye - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: The Borrelia burgdorferi Adenylate Cyclase, CyaB, Is Important for Virulence Factor Production and Mammalian Infection.
    Article Snippet: Seven to 10 μg of RNA was denatured in 2 × RNA load dye (Thermo Fisher) and heated to 65◦C for 15 min, loaded onto a Novex Pre-cast 6% TBE-Urea (8 M) polyacrylamide gel (Thermo Fisher) in 1 × TBE, and run for 45–60 min. RNA was electroblotted at room temperature (10 V for 1 h in 0.5 × TBE) to HybondXL membranes (Amersham).

    Article Title: The Borrelia burgdorferi Adenylate Cyclase, CyaB, Is Important for Virulence Factor Production and Mammalian Infection
    Article Snippet: Seven to 10 μg of RNA was denatured in 2 × RNA load dye (Thermo Fisher) and heated to 65°C for 15 min, loaded onto a Novex Pre-cast 6% TBE-Urea (8 M) polyacrylamide gel (Thermo Fisher) in 1 × TBE, and run for 45–60 min. RNA was electroblotted at room temperature (10 V for 1 h in 0.5 × TBE) to HybondXL membranes (Amersham).

    Incubation:

    Article Title: iRAPs curb antisense transcription in E. coli
    Article Snippet: For northern blotting RNA transcripts, 10 μg of DNAseI-treated (Roche) total RNA was separated by gel electrophoresis in 1% formaldehyde/MOPS denaturing agarose gels in 1× MOPS. .. RNA was denatured in 2X RNA load dye (ThermoFisher Scientific) with incubation for 15 min at 70°C before loading on a gel. .. RNA was transferred to HybondXL membranes by capillary action using 20X SSC buffer for 10–12 h. The membranes were UV cross-linked (150 mJ/cm 2 ) and probed with 5′end γ 32P -labeled DNA oligonucleotide probes using ULTRAhyb-Oligo Hybridization Buffer (Ambion) following the manufacturer's instructions.



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